Bacteriological Profile of Wound Infection and Antibiotic Susceptibility Pattern of Various Isolates in a Tertiary Care Center

Nabina Maharjan; B S Mahawal

doi:10.22502/jlmc.v8i2.367

Bacteriological Profile of Wound Infection and Antibiotic Susceptibility Pattern of Various Isolates in a Tertiary Care Center

Nabina Maharjan Lumbini Medical College and Teaching Hospital https://orcid.org/0000-0003-1873-4646
B S Mahawal Lumbini Medical College and Teaching Hospital

DOI: https://doi.org/10.22502/jlmc.v8i2.367

Keywords: Antibiotic susceptibility test, Extended-Spectrum Beta Lactamases, Methicillin-Resistant Staphylococcus aureus

Abstract

Introduction: Wound infection due to various pathogenic microorganisms and the development of resistance to antibiotics is one of the major problems in medical sector. This study aimed to identify the etiological agents of wound infection along with their antibiotic susceptibility. Methods: A total of 400 wound swab specimens were collected from the patients visiting a tertiary center in western Nepal over a period of six months. Thus, collected specimens were processed in Microbiology laboratory for isolation of causative agents. Antibiotic susceptibility test was performed for entire isolates by Kirby Baur disc diffusion method. Methicillin-Resistant Staphylococcus aureus was detected by cefoxitin disc diffusion test and Extended- Spectrum Beta-Lactamases producing Enterobacteriaeae by Phenotypic confirmatory disc diffusion test as recommended by Clinical and Laboratory Standards Institute. Results: Two hundred and fifty-nine (64.7%) of specimens were infected, giving rise to 269 different isolates. Among these, 166 (61.7%) were gram positive and 101 (37.6%) were gram negative bacteria. Staphylococcus aureus (n = 130, 48.3%) was the most predominant bacteria followed by Escherichia coli (n=44, 16.3%), and Klebsiella pneumoniae (n=23, 8.5%). Gentamicin followed by co-trimoxazole was the most effective among the tested antibiotics for Staphylococcus aureus. Gentamicin and ciprofloxacin were shown effective for isolated gram-negative bacteria. Conclusion: Fifty-eight (44.6%) of total Staphylococcus aureus were Methicillin-Resistant Staphylococcus aureus positive and 16 (20.7%) of total Enterobacteriaceae were Extended-Spectrum Beta-Lactamases producers. The increased prevalence of Methicillin-Resistant Staphylococcus aureus and Extended-Spectrum Beta-Lactamase suggest rational use of antibiotics on the basis of antibiotic sensitivity results.

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Author Biographies

Nabina Maharjan, Lumbini Medical College and Teaching Hospital

Lecturer,

Department of Microbiology.

B S Mahawal, Lumbini Medical College and Teaching Hospital

Professor and Head,

Department of Microbiology.

References

Balchandra MH, Naik SD, Verma PK. Aerobic Bacterial profile of wound infections and its sensitivity pattern at Tertiary care hospital. International Journal of Current Microbiology and Applied Sciences. 2018;7(6):1668-79. DOI: https://doi.org/10.20546/ijcmas.2018.706.198

Brooks GF, Carroll KC, Butel JS, Morse SA. Jawetz, Melnick and Adelberg’s Medical Microbiology. 26th ed. New York: McGraw Hill Lange, New York; 2013. http://microbiology.sbmu.ac.ir/uploads/jawetz_2013__medical_miceobiology.pdf

Ohalete CN, Obi RK, EmeaKoroha MC. Bacteriology of different wound infection and their antimicrobial susceptibility patterns in Imo state Nigeria. World Journal of Pharmacy and Pharmaceutical Sciences. 2012;3(1):1155-72. Available from: https://www.researchgate.net/publication/332849521

Yakha JK, Sharma AR, Dahal N, Lekhak B, Banjara MR. Antibiotic susceptibility pattern of bacterial isolates causing wound infection among the patients visiting B and B hospital. Nepal Journal of Science and Technology. 2014;15(2):91-6. DOI: https://doi.org/10.3126/njst.v15i2.12121

Pant M, Shrestha D, Thapa S. Antibiogram of bacterial species causing skin wound infections. Novel Research in Microbiology Journal. 2018;2(3):53-7. DOI: https://doi.org/10.21608/NRMJ.2018.8153

Mahat P, Manandhar S, Baidya R. Bacteriological Profile of Wound Infection and Antibiotic Susceptibility Pattern of the Isolates. Journal of Microbiology & Experimentation. 2017;4(5):00126. DOI: https://doi.org/10.15406/jmen.2017.04.00126

Cheesbrough M. District laboratory Practice in Tropical Countries. 2nd ed. Cambridge, UK: Cambridge University Press; 2009. DOI: https://doi.org/10.1017/CBO9780511543470

Clinical Laboratory Standarda Institute (CLSI). Performance Standards for Antimicrobial Susceptibility Testing. 29th ed. CLSI supplement M100 Wayne, PA: Clinical and Laboratory Institute; 2019. Available from:https://clsi.org/media/2663/m100ed29_sample.pdf

Sah P, Khanal R, Upadhaya S. Skin and soft tissue infections: Bacteriological profile and antibiotic resistance pattern of isolates. Journal of Universal College Medical Science. 2013;1(3):18-21. DOI: https://doi.org/10.3126/jucms.v1i3.8759

Upreti N, Rayamajhee B, Sherchan SP, Choudhari MK, Banjara MR. Prevalence of methicillin resistant Staphylococcus aureus, multidrug resistant and extended spectrum β- lactamase producing gram negative bacilli causing wound infection at a tertiary care hospital of Nepal. Antimicrobial Resistance and infection control. 2018;7(0):121. DOI: https://doi.org/10.1186/s13756-018-0408-z

KC R, Shrestha A, Sharma VK. Bacteriological study of wound infection and antibiotic susceptibility pattern of the isolates. Nepal Journal of Science and Technology. 2013;14(2):143-7. DOI: https://doi.org/10.3126/njst.v14i2.10428

Khanam RA, Islam MR, Sharif A, Parveen R, Sharmin I, Yusuf MA. Bacteriological profile of pus with antimicrobial sensitivity pattern at a Teaching hospital in Dhaka city. Bangladesh Journal of Infectious Disease. 2018;5(1):10-14. DOI: http://dx.doi.org/10.3329/bjid.v5il.37710

Pandey U, Raut M, Bhattarai S, Bhatt PR, Dahal PR. Bacteriological profile and antibiogram of bacterial isolates from pus sample in tertiary care hospital of Kathmandu. Tribhuvan Uni J Microbiol. 2017;4(1):55-62. DOI: https://doi.org/10.3126/tujm.v4i0.21678

Giri K, Gurung S, Subedi S, Singh A, Adhikari N. Antibiotic susceptibility pattern of bacterial isolates from soft tissues infection among patients visiting Birendra Military Hospital, Chhauni, Kathmandu. Tribhuvan Uni J Microbiol. 2019;6(1):119-26. DOI: https://doi.org/10.3126/tujm.v6i0.26595

Sherchan JB, Gurung P. Antibiotic Susceptibility pattern of bacteriological pathogens isolated from infected lesions. J Nepal Health Res Council. 2018;16(41):446-51. DOI: https://doi.org/10.33314/jnhrc.v16i41.1631

Portigliatti Barbos M, Mognetti B, Pecorara S, Picco W, Veglio V. Decolonization of orthopedic surgical team S. aureus carriers: impact on surgical-site infections. J Orthopaed Traumatol. 2010;11(1):47-9. PMID: 20119678. DOI: https://doi.org/10.1007/s10195-010-0081-3

Harshan KH, Chavan SKD. Prevalence and susceptibility pattern of methicillin resistant Staphylococcus aureus (MRSA) in pus samples at tertiary care hospital in Trivandrum, India. International Journal of current Microbiology and applied sciences. 2015;4(11):718-23. Available from:https://www.ijcmas.com/Archives-36.php

Published

2020-10-29

How to Cite

Maharjan N, Mahawal B. Bacteriological Profile of Wound Infection and Antibiotic Susceptibility Pattern of Various Isolates in a Tertiary Care Center. J Lumbini Med Coll [Internet]. 29Oct.2020 [cited 20May2024];8(2):218-24. Available from: https://jlmc.edu.np/index.php/JLMC/article/view/367

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Vol 8 No 2 (2020): vol:8, No:2

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Original Research Article

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